polyclonal anti igf ii antibody  (R&D Systems)

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 2. Serum IGF2 isoform analysis. (A) WIB analysis of serum IGF2 in eight patients with SFT. Patients #1 to #5 are patients with SFT without hypoglycemia, and patients #10 to #12 are patients with SFT and hypoglycemia. Molecular size markers (in kilodaltons) are indicated by lines on the left. (B) Comparison of big IGF2 production levels between the non-NICTH group and the NICTH group. (C) Comparison of mature IGF2 production levels between the non-NICTH group and the NICTH group. (D) Comparison of the big IGF2/mature IGF2 production-level ratio between the non-NICTH group and the NICTH group. P values were obtained using the t test; P , 0.05 was accepted as significant in all panels.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Comparison

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 3. Comparative analysis of gene-expression levels in SFT using qRT-PCR. (A) IGF2 mRNA expression levels in the non-NICTH group and the NICTH group. (B) PCSK4 mRNA expression levels in the non-NICTH group and the NICTH group. (C) The IGF2/PCSK4 mRNA expression-level ratio in the non-NICTH group and the NICTH group. *P = 0.093, **P = 0.217, ***P = 0.006. P values were obtained using the t test. These values are not statistically significant but tentative as a result of the limited number of samples.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 4. Comparative analysis of protein expression levels in SFT by immunohistochemistry analysis. (A) Representative images of immunohistochemistry for IGF2. (B) Representative images of immunohistochemistry for PCSK4. (C) Comparison of IGF2 expression levels between the non-NICTH group and the NICTH group. (D) Comparison of PCSK4 expression levels between the non-NICTH group and the NICTH group. (E) Comparison of the IGF2/PCSK4 expression-level ratio between the non-NICTH group and the NICTH group. P values were obtained using the t test; (C and E) P , 0.05 was accepted as significant and is indicated in bold.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Expressing, Immunohistochemistry, Comparison

Journal: The Journal of experimental biology

Article Title: Insulin-like growth factor signaling regulates developmental trajectory associated with diapause in embryos of the annual killifish Austrofundulus limnaeus .

doi: 10.1242/jeb.151373

Figure Lengend Snippet: Figure 5. Relative abundance of IGF-I and IGF-II protein in embryos incubated at 25°C

Article Snippet: The samples/standards were neutralized by the addition of 30 μl 200 mmol l-1 Tris (pH 10.0) followed by a 2 h incubation with IGF-II antibody solution (rabbit polyclonal antitrout IGF-II, Lot CA6-PAAA1, 1:3500 dilution, GroPep, Adelaide, Australia).

Techniques: Incubation

Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: The microscopic findings [Hematoxylin and Eosin (H&E) staining and immunohistochemical staining of the resected tumor]. H&E staining (A) Low-power (×40) and (B) high-power (×400) views showed moderate stromal hypercellularity with mild nuclear atypia and mild pleomorphism of the spindle cells. Focal mildly atypical epithelial hyperplasia was also noted. Stromal overgrowth was absent. Immunohistochemical staining of (C) the phyllodes tumor and (D) normal breast tissue using rabbit polyclonal anti-insulin-like growth factor II (IGF-II) antibodies. The tumor cells, but not the normal breast tissue, were diffusely immunopositive for IGF-II.

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Staining, Immunohistochemical staining

Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: Western blotting of high-molecular-weight IGF-II. Lane 1, serum from a healthy control; lane 2, serum from the patient collected preoperatively; lane 3, serum obtained 3 days after the resection of the tumor; lane 4, phyllodes tumor tissue. Large amounts of high-molecular-weight IGF-II were detected in the serum collected preoperatively (lane 2) and in the tumor tissue (lane 4) but not in serum collected postoperatively (lane 3) or a serum sample from a healthy control (lane 1).

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Western Blot, High Molecular Weight, Control

Journal: Oncotarget

Article Title: IGF-II induced by hepatitis B virus X protein regulates EMT via SUMO mediated loss of E-cadherin in mice

doi: 10.18632/oncotarget.10922

Figure Lengend Snippet: A. and C. Relative mRNA expression of IGF-II in WT and HBx mouse livers (A) Mean ± SD ; * P< 0.05, ** P< 0.01, n =3, HepG2-Mock and HepG2-HBx cells (C) Mean + SD ; * P< 0.05, n =5-8. B. Immunohistochemistry images of 3-week-old WT and HBx-mouse livers stained with IGF-II. Original magnifications 40X. Data are representative of 3-5 mice per group. D. Secreted IGF-II protein levels from HepG2-Mock and HBx cells were measured. Mean ± SD ; * P< 0.05, n =6.

Article Snippet: Samples were incubated overnight at 4 °C with anti-IGF-II rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology, CA, USA).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: IGF-II induced by hepatitis B virus X protein regulates EMT via SUMO mediated loss of E-cadherin in mice

doi: 10.18632/oncotarget.10922

Figure Lengend Snippet: Mean ± SD ; * P< 0.05, n =6. B. Cell growth was measured in HepG2-Mock cells treated with conditioned medium from HepG2-Mock (CM-Mock), and HBx (CM-HBx). Mean ± SD ; * P < 0.05, n=6. C. Cell numbers were quantified in HepG2-Mock and HBx cells with or without anti-IGF-II. Mean ± SD ; ** P< 0.01, n =4. D. Multilayer growth of HepG2-HBx cells were reversed to monolayer growth with anti-IGF-II.

Article Snippet: Samples were incubated overnight at 4 °C with anti-IGF-II rabbit polyclonal antibody (1:200; Santa Cruz Biotechnology, CA, USA).

Techniques: